SYNTHETIC ANTIGENS AS IMMUNOGENS .4. SPECIFICITY OF ANTISERA DEVELOPED TO GAL BETA-1-3(GLCNAC BETA-1-6)GALNAC AND GLCNAC BETA-1-6GALNAC

被引：3
|
作者：
MATTA, KL
PISKORZ, CF
ABBAS, SA
DIAKUN, KR
机构：
[1] Department of Gynecologic Oncology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY
来源：
IMMUNOLOGICAL INVESTIGATIONS | 1991年 / 20卷 / 5-6期
关键词：
D O I：
10.3109/08820139109082633
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Antisera to the chemically synthesized trisaccharide, Gal beta-1-3(GlcNAc beta-1-6)GalNAc, and disaccharide, GlcNAc beta-1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically synthesized oligosaccharides. The anti-trisaccharide antibody showed no reactivity to T antigenic structures which bear the Gal beta-1-3GalNAc moiety. The immunodominant area of the Gal beta-1-3(GlcNAc beta-1-6)GalNAc was contained in the GlcNAc beta-1-6GalNAc region of the molecule. The reactivity pattern of the anti-trisaccharide antibody, although directed to the disaccharide, did not exactly duplicate the reactivity pattern of the anti-disaccharide.
引用
收藏
页码：525 / 537
页数：13
相关论文
共 50 条
  • [1] MONOCLONAL IGM IN 2 PATIENTS WITH MOTOR NEURON DISEASE BIND TO THE CARBOHYDRATE ANTIGENS GAL(BETA-1-3)GALNAC AND GAL(BETA-1-3)GLCNAC
    ITO, H
    LATOV, N
    JOURNAL OF NEUROIMMUNOLOGY, 1988, 19 (03) : 245 - 253
  • [2] ENZYMATIC INVITRO SYNTHESIS OF RADIOLABELED PENTASACCHARIDES GLCNAC-BETA-1-3(GAL-BETA-1-4GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC/GLC AND THE ISOMERIC GAL-BETA-1-4GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC/GLC
    RENKONEN, O
    LEPPANEN, A
    NIEMELA, R
    VILKMAN, A
    HELIN, J
    PENTTILA, L
    MAAHEIMO, H
    SEPPO, A
    SUOPANKI, J
    BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1992, 70 (01): : 86 - 89
  • [3] ACTION OF RAT-LIVER GAL-BETA-1-4GLCNAC ALPHA(2-6)-SIALYLTRANSFERASE ON MAN-BETA-1-4GLCNAC-BETA-OME, GALNAC-BETA-1-4GLCNAC-BETA-OME, GLC-BETA-1-4GLCNAC-BETA-OME AND GLCNAC-BETA-1-4GLCNAC-BETA-OME AS SYNTHETIC SUBSTRATES
    HOKKE, CH
    VANDERVEN, JGM
    KAMERLING, JP
    VLIEGENTHART, JFG
    GLYCOCONJUGATE JOURNAL, 1993, 10 (01) : 82 - 90
  • [4] MUCIN SYNTHESIS .6. UDP-GLCNAC-GALNAC-R BETA-3-N-ACETYLGLUCOSAMINYLTRANSFERASE AND UDP-GLCNAC-GLCNAC-BETA-1-3GALNAC-R (GLCNAC TO GALNAC)
    BROCKHAUSEN, I
    MATTA, KL
    ORR, J
    SCHACHTER, H
    BIOCHEMISTRY, 1985, 24 (08) : 1866 - 1874
  • [5] UDP-GLCNAC - GAL-BETA-3GALNAC-MUCIN - (GLCNAC-]GALNAC) BETA-6-N-ACETYLGLUCOSAMINYLTRANSFERASE AND UDP-GLCNAC - GAL-BETA-3(GLCNAC-BETA-6) GALNAC-MUCIN (GLCNAC-]GAL)BETA-3-N-ACETYLGLUCOSAMINYLTRANSFERASE FROM SWINE TRACHEA EPITHELIUM
    SANGADALA, S
    SIVAKAMI, S
    MENDICINO, J
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 1991, 101 (02) : 125 - 143
  • [6] ANTIGENIC STUDIES ON AN ENZYMATICALLY SIALYLATED CARBOHYDRATE - NEUAC(ALPHA-2-3)GAL(BETA-1-3)[GLCNAC(BETA-6)]GALNAC
    HANDLEY, DJ
    MATTA, KL
    PIVER, MS
    DIAKUN, KR
    IMMUNOLOGICAL INVESTIGATIONS, 1991, 20 (01) : 21 - 32
  • [7] ESCHERICHIA-COLI BETA-GALACTOSIDASE UNEXPECTEDLY CLEAVES THE HEXASACCHARIDE GAL-BETA-1-4GLCNAC-BETA-1-3(GAL-BETA-1-4GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC WITHOUT BRANCH SPECIFICITY
    RENKONEN, O
    HELIN, J
    VAINIO, A
    NIEMELA, R
    PENTTILA, L
    HILDEN, P
    BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1990, 68 (7-8): : 1032 - 1036
  • [8] A NOVEL BETA-N-ACETYLGLUCOSAMINIDASE ACTIVITY IN HOG GASTRIC-MUCOSAL MICROSOMES - PREFERENTIAL HYDROLYSIS OF TERMINAL GLCNAC-BETA-1-3 LINKAGES IN GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC, BUT GLCNAC-BETA-1-6 LINKAGES IN GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL
    HELIN, J
    SEPPO, A
    LEPPANEN, A
    PENTTILA, L
    MAAHEIMO, H
    NIEMELA, R
    LAURI, S
    RENKONEN, O
    FEBS LETTERS, 1993, 335 (02) : 280 - 284
  • [9] PROCESSING OF O-GLYCAN CORE-1, GAL-BETA-1-3GALNAC-ALPHA-R - SPECIFICITIES OF CORE-2, UDP-GLCNAC-GAL-BETA-1-3GALNAC-R (GLCNAC TO GALNAC) BETA-6-N-ACETYLGLUCOSAMINYLTRANSFERASE AND CMP-SIALIC ACID - GAL-BETA-1-3GALNAC-R ALPHA-3-SIALYLTRANSFERASE
    KUHNS, W
    RUTZ, V
    PAULSEN, H
    MATTA, KL
    BAKER, MA
    BARNER, M
    GRANOVSKY, M
    BROCKHAUSEN, I
    GLYCOCONJUGATE JOURNAL, 1993, 10 (05) : 381 - 394
  • [10] WHEAT-GERM-AGGLUTININ CHROMATOGRAPHY OF GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL AND GLCNAC-BETA-1-3(GLCNAC-BETA-1-6)GAL-BETA-1-4GLCNAC, OBTAINED BY INVITRO SYNTHESIS AND BY PARTIAL CLEAVAGE OF TERATOCARCINOMA POLY-N-ACETYLLACTOSAMINOGLYCANS
    SEPPO, A
    PENTTILA, L
    MAKKONEN, A
    LEPPANEN, A
    NIEMELA, R
    JANTTI, J
    HELIN, J
    RENKONEN, O
    BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1990, 68 (01): : 44 - 53
12345