USE OF A NOVEL AGAROSE GEL-DIGESTING ENZYME FOR EASY AND RAPID PURIFICATION OF PCR-AMPLIFIED DNA FOR SEQUENCING

被引：0

作者：

GOLD, P

机构：

来源：

BIOTECHNIQUES | 1992年 / 13卷 / 01期

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D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of a novel gel-digesting enzyme preparation provides an easy, rapid and convenient method to quantitatively recover PCR-amplified DNA from low melting point agarose gels. The PCR products purified using this method were readily sequenced and yielded good and unambiguous sequence data.

引用

页码：132 / 134

页数：3

共 8 条

[1] Detection of a single base exchange in PCR-amplified DNA fragments using agarose gel electrophoresis containing bisbenzimide-PEG
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[2] High-speed gel microelectrophoresis, a new and easy approach for detection of PCR-amplified microbial DNA from environmental and clinical samples in microgels using conventional equipment
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[3] The use of multiple displacement amplified DNA as a control for methylation specific PCR, pyrosequencing, bisulfite sequencing and methylation-sensitive restriction enzyme PCR
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[4] DENATURING GRADIENT GEL-ELECTROPHORESIS AND DIRECT SEQUENCING OF PCR AMPLIFIED GENOMIC DNA - A RAPID AND RELIABLE DIAGNOSTIC-APPROACH TO BETA THALASSEMIA
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HARTEVELD, CL
VANHEEREN, H
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[5] DENATURING GRADIENT GEL-ELECTROPHORESIS (DGGE) AND DIRECT SEQUENCING OF PCR AMPLIFIED GENOMIC DNA - RAPID AND RELIABLE IDENTIFICATION OF HELIANTHUS-ANNUUS L CULTIVARS
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[6] Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments
Kowalchuk, GA
Stephen, JR
DeBoer, W
Prosser, JI
Embley, TM
Woldendorp, JW
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (04) : 1489 - 1497
[7] Use of a novel DNA melting profile assay for the identification of PCR-amplified genomic sequences encoding for variant-specific surface proteins from the clonal GS/M-83-H7 line of Giardia lamblia
Bienz, M
Siles-Lucas, M
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PARASITOLOGY RESEARCH, 2001, 87 (12) : 1011 - 1015
[8] Use of a novel DNA melting profile assay for the identification of PCR-amplified genomic sequences encoding for variant-specific surface proteins from the clonal GS/M-83-H7 line of Giardia lamblia
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