ISOLATION OF A POPLAR AND AN ARABIDOPSIS-THALIANA DIHYDRODIPICOLINATE SYNTHASE CDNA CLONE

被引：25
|
作者：
VAUTERIN, M
JACOBS, M
机构：
[1] Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, St.-Genesius Rode, B-1640
来源：
PLANT MOLECULAR BIOLOGY | 1994年 / 25卷 / 03期
关键词：
ARABIDOPSIS THALIANA; DIHYDRODIPICOLINATE SYNTHASE; FUNCTIONAL COMPLEMENTATION; POLYMERASE CHAIN REACTION; POPLAR;
D O I：
10.1007/BF00043882
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A poplar DHDPS cDNA clone has been isolated by functional rescue of the dapA-deficient AT997 mutant of Escherichia coli. By sequence comparison between the polar and maize DHDPS cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) on Avabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate an Arabidopsis DHDPS genomic and cDNA clone.
引用
收藏
页码：545 / 550
页数：6
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