COMPARISON OF SEROLOGICAL TESTS FOR DIAGNOSIS OF CAPRINE BRUCELLOSIS

The efficacy of dot-enzyme linked immunosorbent assay (dot-ELISA) and indirect haemolysis test (IIILT) was compared with conventional serological tests, viz. standard tube agglutination test (STAT), Rose Bengal plate test (RBPT), and complement fixation test (CFT) for diagnosing caprine brucellosis. Our of 75 field serum samples, none could prove positive to RBPT, STAT and IHLT, whereas, 2 (2.26%) and 1 (1.33%) were positive to CFT and IgG dot-ELISA respectively. The relative sensitive and relative specificity in comparison with CFT was 50% and 100% for dot-ELISA. The RBPT, STAT and IHLT could not prove sensitive, whereas, all these tests were relatively specific when compared with CFT. In experimentally infected gears dot-ELISA could detect positive animals earlier to all other tests compared. The dot-ELISA war more sensitive, economical and a rapid test for screening caprine brucellosis in field conditions.