DIFFERENTIATION OF AUJESZKYS-DISEASE VIRUS-STRAINS ISOLATED IN POLAND USING DNA BIOTINYLATED PROBES

被引：0
|
作者：
KOCHAN, G [1 ]
LIPOWSKI, A [1 ]
FICINSKA, J [1 ]
SZEWCZYK, B [1 ]
机构：
[1] NATL VET RES INST,DEPT SWINE DIS,PL-24100 PULAWY,POLAND
来源：
ACTA VETERINARIA HUNGARICA | 1994年 / 42卷 / 2-3期
关键词：
AUJESZKYS DISEASE VIRUS; CLINICAL OUTBREAK; ISOLATE; DIFFERENTIATION; DNA PROBE; RESTRICTION FRAGMENT ANALYSIS;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of this study was to compare 17 different Aujeszky's disease virus (ADV) isolates from clinical outbreaks of AD by using DNA biotinylated probes. All isolates were collected in Poland between 1984 and 1991. The restriction fragment pattern (RFP) analysis done by hybridization to NIA-3 DNA biotinylated probe indicated that all Polish ADV field strains can be classified as type I of Suid herpesvoirus 1. Hybridization with BamHI fragment 7 and gI gene biotinylated probes revealed an unusual heterogeneity of BamHI fragment 7 in almost 50% of strains isolated in Poland. The nature of the molecular changes in this fragment will be discussed.
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页码：369 / 376
页数：8
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