A PRESSURE JUMP APPARATUS WITH OPTICAL DETECTION

被引：13
作者：
GOLDSACK, DE
HURST, RE
LOVE, J
机构：
[1] Fisheries Research Board of Canada, Vancouver Laboratory, Vancouver 8, BC
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期
关键词：
D O I：
10.1016/0003-2697(69)90179-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A pressure jump apparatus with optical detection for studying fast transient chemical reactions has been described. The instrument can be used in the time region of 100 μsec to 5 sec. Preliminary results on the kinetics of isomerization of bovine serum albumin at alkaline pH are reported. Two well-separated relaxation times were found. The slow relaxation time (100 to 200 msec) was concentration independent and was attributed, there-fore, to the isomerization of the protein. The fast relaxation time (2 to 10 msec) was dependent on the concentration of protein and indicator (phenol red), and was associated with the binding of the dye to the protein which is coupled to the isomerization of the protein-dye complex. © 1969.
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页码：273 / &
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