PROTEIN HISTIDINE PHOSPHATASE-ACTIVITY IN RAT-LIVER AND SPINACH LEAVES

被引：16
|
作者：
MATTHEWS, HR [1 ]
MACKINTOSH, C [1 ]
机构：
[1] UNIV DUNDEE,DEPT BIOCHEM,MRC,PROT PHOSPHORYLAT LAB,DUNDEE DD1 4HN,SCOTLAND
来源：
FEBS LETTERS | 1995年 / 364卷 / 01期
关键词：
PHOSPHOPROTEIN PHOSPHATASE; PROTEIN HISTIDINE PHOSPHATASE; PHOSPHOHISTIDINE; RAT; SPINACH;
D O I：
10.1016/0014-5793(95)00353-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Whole cell extracts from rat liver or spinach leaves contain divalent ion-independent protein histidine phosphatase activity due to phosphatases of the PP1/PP2A family. In the rat liver extract, almost all the activity was found in the PP1, PP2A(1) and PP2A(2) peaks. In the spinach leaf extract, four phosphorylase phosphatase activity peaks were resolved - three containing PP1 and one containing PP2A - and all showed histidine phosphatase activity. Thus, protein histidine phosphatase activity is expressed in the cytosolic forms of protein phosphatases of the PP1/PP2A family in mammalian and plant cells.
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页码：51 / 54
页数：4
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