AFB1 Occurrence and Aflatoxin Biosynthesis Genes in Wheat Derivatives

被引:0
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作者
Mahmoud, Mohamed A. [1 ]
Abd-El-Aziz, Abeer R. M. [2 ]
机构
[1] Agr Res Ctr, Plant Pathol Res Inst, Giza, Egypt
[2] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia
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中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The incidence of AFB1 (B1) was determined in 72 wheat derivatives samples (Biscuits, bread, cornflakes, flour, macaroni and wheat bran. Positive sample for biscuits, bread and flour (16.7%), cornflakes (8.33%), macaroni (33.33%) and wheat bran (50%). Sixteen samples were contaminated with B1 ranging from 0.21 to 2.17 mu g/kg. PCR was used for the detection of B1 contamination based on aflatoxin biosynthetic genes af1R, af1S, af1D, af1M and af1P. The interpretation of the results revealed that PCR is a rapid and sensitive method.
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页码:3641 / 3645
页数:5
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