NOE AND 2-DIMENSIONAL CORRELATED H-1-NMR SPECTROSCOPY OF CYTOCHROME-C' FROM CHROMATIUM-VINOSUM

被引：36

作者：

BANCI, L ^{[1
]}

BERTINI, I ^{[1
]}

TURANO, P ^{[1
]}

OLIVER, MV ^{[1
]}

机构：

[1] UNIV FLORENCE,DEPT CHEM,VIA GINO CAPPONI 7,I-50121 FLORENCE,ITALY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 204卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1992.tb16611.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

H-1 two-dimensional (nuclear Overhauser effect spectroscopy (NOESY) and two-dimensional correlated spectroscopy (COSY) spectra of cytochrome c' from Chromatium vinosum have been obtained. The protein is of medium size (M(r) 28 000), essentially high spin (S = 5/2) although some quantum mechanical spin admixing with S = 3/2 may be present. Under these circumstances NOESY cross peaks have been revealed between geminal protons (alpha-CH2 propionate and beta-CH2 protons of the bound histidine) and between alpha-CH2 propionate protons and the heme methyl groups. COSY maps have confirmed the geminal nature of the proton pairs, even with a linewidth as large as 900 Hz; the J value is about 12 Hz. This assignment has rationalized on a sound basis the biochemical behavior of this protein with pH and has showed the utility of this kind of spectroscopy for the other cytochromes c' structures and analogous systems.

引用

页码：107 / 112

页数：6

共 48 条

[1] AMINO-ACID-SEQUENCES OF BACTERIAL CYTOCHROMES-C' AND C-556 [J].

AMBLER, RP ;

BARTSCH, RG ;

DANIEL, M ;

KAMEN, MD ;

MCLELLAN, L ;

MEYER, TE ;

VANBEEUMEN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11) :6854-6857

[2] 2-DIMENSIONAL SPECTROSCOPY - APPLICATION TO NUCLEAR MAGNETIC-RESONANCE [J].

AUE, WP ;

BARTHOLDI, E ;

ERNST, RR .

JOURNAL OF CHEMICAL PHYSICS, 1976, 64 (05) :2229-2246

[3] H-1-NMR SPECTRA OF OXIDIZED HIGH-POTENTIAL IRON SULFUR PROTEIN (HIPIP) FROM RHODOCYCLUS-GELATINOSUS - A MODEL FOR OXIDIZED HIPIPS [J].

BANCI, L ;

BERTINI, I ;

BRIGANTI, F ;

LUCHINAT, C ;

SCOZZAFAVA, A ;

OLIVER, MV .

INORGANIC CHEMISTRY, 1991, 30 (24) :4517-4524

[4] H-1 NOE STUDIES ON DICOPPER(II) DICOBALT(II) SUPEROXIDE-DISMUTASE [J].

BANCI, L ;

BERTINI, I ;

LUCHINAT, C ;

PICCIOLI, M ;

SCOZZAFAVA, A ;

TURANO, P .

INORGANIC CHEMISTRY, 1989, 28 (26) :4650-4656

[5] COMPARATIVE H-1-NMR STUDY OF FERRIC LOW-SPIN CYTOCHROME-C PEROXIDASE AND HORSERADISH-PEROXIDASE [J].

BANCI, L ;

BERTINI, I ;

TURANO, P ;

FERRER, JC ;

MAUK, AG .

INORGANIC CHEMISTRY, 1991, 30 (24) :4510-4516

[6] PROTON NMR INVESTIGATION INTO THE BASIS FOR THE RELATIVELY HIGH REDOX POTENTIAL OF LIGNIN PEROXIDASE [J].

BANCI, L ;

BERTINI, I ;

TURANO, P ;

TIEN, M ;

KIRK, TK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :6956-6960

[7] PH VARIATION OF MIDPOINT POTENTIAL FOR 3 PHOTOSYNTHETIC BACTERIAL CYTOCHROMES-C' - A LINK BETWEEN PHYSICAL AND FUNCTIONAL-PROPERTIES [J].

BARAKAT, R ;

STREKAS, TC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 679 (03) :393-399

[8]

Bartsch R. G., 1971, METHOD ENZYMOL, V23, P344, DOI [10.1016/S0076-6879(71)23110-4, DOI 10.1016/S0076-6879(71)23110-4]

[9] APPLICATIONS OF COSY TO PARAMAGNETIC HEME-CONTAINING SYSTEMS [J].

BERTINI, I ;

CAPOZZI, F ;

LUCHINAT, C ;

TURANO, P .

JOURNAL OF MAGNETIC RESONANCE, 1991, 95 (02) :244-252

[10] H-1-NMR STUDIES OF CHROMATIUM-VINOSUM CYTOCHROME-C' [J].

BERTINI, I ;

BRIGANTI, F ;

MONNANNI, R ;

SCOZZAFAVA, A ;

CARLOZZI, P ;

MATERASSI, R .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 282 (01) :84-90

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