CORE HISTONE ACETYLATION DOES NOT BLOCK LINKER HISTONE BINDING TO A NUCLEOSOME INCLUDING A XENOPUS-BOREALIS 5-S RIBOSOMAL-RNA GENE

We demonstrate that acetylation of core histone tails does not significantly impair the ability of linker histones to bind preferentially and asymmetrically to a defined sequence mononucleosome core reconstituted in vitro. Thus a simple inhibition mechanism cannot explain models whereby acetylation is causal for the observed reduction in the linker histone content in chromatin. Other models to explain this correlation are discussed.