CHARACTERIZATION OF LOW POPULATED PEPTIDE HELICAL STRUCTURES IN SOLUTION BY MEANS OF NMR PROTON CONFORMATIONAL SHIFTS

被引：34

作者：

BRUIX, M

PERELLO, M

HERRANZ, J

RICO, M

NIETO, JL

机构：

[1] CSIC, INST ESTRUCT MAT, SERRANO 119, E-28006 MADRID, SPAIN

[2] CNRS, CTR BIOPHYS MOLEC, F-45071 ORLEANS 2, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90623-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A NOE independent NMR method is proposed to characterize unambiguosly residues involved in low populated isolated peptide helices. The method is based on the comparison of amide and Hα chemical shift changes originated upon the addition of stabilizing or denaturing agents with true helical conformational shifts that have been measured for the first time using an isolated model peptide helix, the one formed by Ac-(Leu-Lys-Lys-Leu)3-NHEt in aqueous solution. © 1990.

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页码：1009 / 1014

页数：6

相关论文

共 20 条

[1] HYDROGEN-BONDING IN GLOBULAR-PROTEINS [J].

BAKER, EN ;

HUBBARD, RE .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1984, 44 (02) :97-179

[2] SEEDING PROTEIN FOLDING [J].

BALDWIN, RL .

TRENDS IN BIOCHEMICAL SCIENCES, 1986, 11 (01) :6-9

[3] ALPHA-CONFORMATION AND BETA-CONFORMATION OF WATER-SOLUBLE SEQUENTIAL ISO POLYPEPTIDES [J].

BARBIER, B ;

PERELLO, M ;

BRACK, A .

COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1988, 53 (11B) :2825-2832

[4] SOLVENT-INDUCED DISTORTIONS AND THE CURVATURE OF ALPHA-HELICES [J].

BLUNDELL, T ;

BARLOW, D ;

BORKAKOTI, N ;

THORNTON, J .

NATURE, 1983, 306 (5940) :281-283

[5]

Bradbury E. M., 1973, Pure and Applied Chemistry, V36, P53, DOI 10.1351/pac197336010053

[6] DIVERSITY OF THE GRAMICIDIN A SPATIAL STRUCTURE - TWO-DIMENSIONAL H-1-NMR STUDY IN SOLUTION [J].

BYSTROV, VF ;

ARSENIEV, AS .

TETRAHEDRON, 1988, 44 (03) :925-940

[7] PEPTIDE-BOND DISTORTIONS AND THE CURVATURE OF ALPHA-HELICES [J].

CHAKRABARTI, P ;

BERNARD, M ;

REES, DC .

BIOPOLYMERS, 1986, 25 (06) :1087-1093

[8]

DEGRADO WF, 1988, ADV PROTEIN CHEM, V39, P51

[9] FOLDING OF A PEPTIDE CORRESPONDING TO THE ALPHA-HELIX IN BOVINE PANCREATIC TRYPSIN-INHIBITOR [J].

GOODMAN, EM ;

KIM, PS .

BIOCHEMISTRY, 1989, 28 (10) :4343-4347

[10] DISTRIBUTION OF CHEMICAL-SHIFTS IN H-1 NUCLEAR MAGNETIC-RESONANCE SPECTRA OF PROTEINS [J].

GROSS, KH ;

KALBITZER, HR .

JOURNAL OF MAGNETIC RESONANCE, 1988, 76 (01) :87-99