RAPID SEPARATION OF THE ALPHA,BETA,G-GAMMA AND A-GAMMA HUMAN GLOBIN CHAINS BY REVERSED-PHASE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：56
作者：
CONGOTE, LF
BENNETT, HPJ
SOLOMON, S
机构：
[1] MCGILL UNIV,DEPT BIOCHEM,MONTREAL H3C 3G1,QUEBEC,CANADA
[2] MCGILL UNIV,DEPT OBSTET & GYNECOL,MONTREAL H3C 3G1,QUEBEC,CANADA
[3] ROYAL VICTORIA HOSP,MONTREAL H3A 1A1,QUEBEC,CANADA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 03期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1016/0006-291X(79)91856-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The main human globin chains present in cord blood hemoglobins, α,β,Gγ and Aγ, can be separated in 45 minutes by reversedphase high pressure liquid chromatography. The chains were identified by carboxymethyl cellulose chromatography and partial amino acid analysis of the cyanogen bromide fragments of the two γ chains. The purification of cyanogen bromide fragments and the separation and quantitation of their dansylated amino acids were accomplished using a similar system to that used for the separation of the globin chains. These results show the potential of this type of chromatography for the analytical and semi-preparative analysis of globin chains and the large advantages over conventional chromatographic techniques. © 1979.
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页码：851 / 858
页数：8
相关论文
共 6 条
[1]   PRENATAL DIAGNOSIS OF HEMOGLOBINOPATHIES - REVIEW OF 15 CASES [J].
ALTER, BP ;
MODELL, CB ;
FAIRWEATHER, D ;
HOBBINS, JC ;
MAHONEY, MJ ;
FRIGOLETTO, FD ;
SHERMAN, AS ;
NATHAN, DG .
NEW ENGLAND JOURNAL OF MEDICINE, 1976, 295 (26) :1437-1443
[2]   G-GAMMA-GLOBIN AND A-GAMMA-GLOBIN CHAINS SEPARATION AND QUANTITATION BY ISOELECTRIC-FOCUSING [J].
COMI, P ;
GIGLIONI, B ;
OTTOLENGHI, S ;
GIANNI, AM ;
RICCO, G ;
MAZZA, U ;
SAGLIO, G ;
CAMASCHELLA, C ;
PICH, PG ;
GIANAZZA, E ;
RIGHETTI, PG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 87 (01) :1-8
[3]  
Gray W R, 1972, Methods Enzymol, V25, P121, DOI 10.1016/S0076-6879(72)25010-8
[4]   PRESENT STATUS OF HETEOGENEITY OF FETAL HEMOGLOBIN IN BETA-THALASSEMIA - ATTEMPT TO UNIFY SOME OBSERVATIONS IN THALASSEMIA AND RELATED CONDITIONS [J].
HUISMAN, THJ ;
SCHROEDER, WA ;
EFREMOV, GD ;
DUMA, H ;
MLADENOVSKI, B ;
HYMAN, CB ;
RACHMILEWITZ, EA ;
BOUVER, N ;
MILLER, A ;
BRODIE, A ;
SHELTON, JR ;
SHELTON, JB ;
APELL, G .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1974, 232 (MAY24) :107-124
[5]  
Huisman THJ, 1977, HEMOGLOBINOPATHIES T
[6]   SEPARATION OF DANSYL AMINO-ACIDS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
WILKINSON, JM .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1978, 16 (11) :547-552
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