RETENTION REPRODUCIBILITY OF BASIC DRUGS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON A SILICA COLUMN WITH A METHANOL HIGH-PH BUFFER ELUENT - EFFECT OF OPERATING-CONDITIONS ON SEPARATIONS USING AN ORGANIC BUFFER

被引：20
|
作者：
SMITH, RM [1 ]
WESTLAKE, JP [1 ]
GILL, R [1 ]
OSSELTON, MD [1 ]
机构：
[1] CENT RES & SUPPORT ESTABL,HOME OFF FORENS SCI SERV,READING RG7 4PN,BERKS,ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY | 1990年 / 514卷 / 02期
关键词：
D O I：
10.1016/S0021-9673(01)89382-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Basic drugs can be separated by high-performance liquid chromatography on silica using a methanol-aqueous pH-10 buffer eluent prepared from 3-(cyclohexylamino)-1-propanesulphonic acid and sodium 3-(cyclohexylamino)-2-hydroxyl-1-propanesulphonate. The buffer could be reproducibly prepared. The effects of small changes in the buffer and eluent composition and in the operating temperature on the relative retentions of the different groups of drugs were determined. © 1990.
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页码：97 / 109
页数：13
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