NUCLEAR MAGNETIC-RESONANCE STUDIES ON TRANSFER RIBONUCLEIC-ACID - ASSIGNMENT OF AU TERTIARY RESONANCES

被引：32

作者：

HURD, RE ^{[1
]}

REID, BR ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BIOCHEM,RIVERSIDE,CA 92521

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 18期

关键词：

D O I：

10.1021/bi00585a025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hydrogen-bonded ring NH nuclear magnetic resonance (NMR) spectra of several transfer ribonucleic acid (tRNA) species have been examined with particular emphasis on the extreme low-field portion. Between -13.8 and -15 ppm there are two extra resonances which are not derived from cloverleaf base pairs. A combined approach involving un-dermodified tRNAs, chemical modification, and hairpin fragment studies has assigned the T54-A58 resonance at -14.3 ppm in yeast tRNAPhe and Escherichia coli tRNA1Val; the U8-A14 resonance has been assigned at -14.3 ppm, and the s4U8-A14 resonance in bacterial tRNAs has been assigned at -14.9 ppm. The T54-A58 resonance shifts between -14.3 and -13.8 ppm depending on the surrounding nucleotide sequence in the ribothymidine loop. © 1979, American Chemical Society. All rights reserved.

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页码：4005 / 4011

页数：7

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共 34 条

[1] RING CURRENT SHIELDING EFFECTS IN NUCLEIC-ACID DOUBLE HELICES [J].

ARTER, DB ;

SCHMIDT, PG .

NUCLEIC ACIDS RESEARCH, 1976, 3 (06) :1437-1447

[2] NMR EVIDENCE FOR COMMON TERTIARY STRUCTURE BASE-PAIRS IN YEAST AND ESCHERICHIA-COLI TRANSFER-RNA [J].

BOLTON, PH ;

KEARNS, DR .

NATURE, 1975, 255 (5506) :347-349

[3] QUANTIFICATION OF SECONDARY AND TERTIARY STRUCTURE BASE-PAIRS IN ESCHERICHIA-COLI TRANSFER-RNA-1VAL [J].

BOLTON, PH ;

KEARNS, DR .

NATURE, 1976, 262 (5567) :423-424

[4] QUANTITATIVE-DETERMINATION OF NUMBER OF SECONDARY AND TERTIARY STRUCTURE BASE-PAIRS IN TRANSFER-RNA IN SOLUTION [J].

BOLTON, PH ;

JONES, CR ;

BASTEDOLERNER, D ;

WONG, KL ;

KEARNS, DR .

BIOCHEMISTRY, 1976, 15 (20) :4370-4377

[5] MOLECULAR MECHANISM OF THERMAL UNFOLDING OF ESCHERICHIA-COLI FORMYLMETHIONINE TRANSFER-RNA [J].

CROTHERS, DM ;

COLE, PE ;

HILBERS, CW ;

SHULMAN, RG .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 87 (01) :63-&

[6] CHANGES IN TERTIARY STRUCTURE ACCOMPANYING A SINGLE BASE CHANGE IN TRANSFER-RNA - PROTON MAGNETIC-RESONANCE AND AMINOACYLATION STUDIES OF ESCHERICHIA-COLI TRANSFER-RNA METF1 AND TRANSFER-RNA METF3 AND THEIR SPIN-LABELED (S4U8) DERIVATIVES [J].

DANIEL, WE ;

COHN, M .

BIOCHEMISTRY, 1976, 15 (18) :3917-3924

[7] PROTON NUCLEAR MAGNETIC-RESONANCE OF SPIN-LABELED ESCHERICHIA-COLI TRANSFER-RNA-F1MET [J].

DANIEL, WE ;

COHN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (07) :2582-2586

[8] INTERMOLECULAR NUCLEAR SHIELDING VALUES FOR PROTONS OF PURINES AND FLAVINS [J].

GIESSNERPRETTRE, C ;

PULLMAN, B .

JOURNAL OF THEORETICAL BIOLOGY, 1970, 27 (01) :87-+

[9] SEPARATION OF SOLUBLE RIBONUCLEIC ACIDS ON BENZOYLATED DIETHYLAMINOETHYLCELLULOSE [J].

GILLAM, I ;

MILLWARD, S ;

BLEW, D ;

VONTIGER.M ;

WIMMER, E ;

TENER, GM .

BIOCHEMISTRY, 1967, 6 (10) :3043-&

[10] SEPARATION OF TRANSFER RIBONUCLEIC-ACID BY SEPHAROSE CHROMATOGRAPHY USING REVERSE SALT GRADIENTS [J].

HOLMES, WM ;

HURD, RE ;

REID, BR ;

RIMERMAN, RA ;

HATFIELD, GW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (03) :1068-1071

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