Antagonistic Activity of Nocardia brasiliensis PTCC 1422 Against Isolated Gram-negative Bacteria from Urinary Tract Infections

The nocardiae are bacteria belonging to the aerobic actinomycetes. They are partially acid fast, Gram-positive, branching filamentous bacteria that are found ubiquitously in soil, fresh water and marine water. Bacterial urinary tract infections are frequent in the outpatient as well as in the nosocomial setting. The common bacteria from UTI, were isolated from hospital and laboratory samples. The present study was designed to isolate Gram- negative bacteria from urinary tract infections and evaluate Nocardia brasiliensis PTCC 1422 antimicrobial activity against pathogenic bacteria. Bacterial isolates were identified as Acinetobater spp. and Pseudomonas aeruginosa. The cell free supernatants of the N. brasiliensis were able to inhibit the growth of all human pathogens (Acinetobater spp. and Pseudomonas aeruginosa) isolated in this study in Well diffusion method. The isolates also showed very promising activities against multi drug resistant human pathogens.