ANTI-HORMONAL AGENTS .6. DIRECT PLASMA ANALYSIS OF TAMOXIFEN BY HPLC USING AN ONLINE ISRP EXTRACTION CARTRIDGE

HPLC analysis of the anti-estrogen tamoxifen can be achieved by direct injection of diluted plasma samples without pre-treatment. The method uses an additional HPLC pump and two injectors and involves in-line filtration and sample clean up on a loading loop cartridge containing an internal surface reverse phase packing, followed by elution on an ODS-silica column. The minimization of sample handling and avoidance of extraction and adsorption losses result in a very rapid and accurate procedure suitable for routine assays. © 1990, Taylor & Francis Group, LLC. All rights reserved.