AN INVITRO MODEL FOR SCREENING ANTILEISHMANIAL DRUGS - THE HUMAN LEUKEMIA MONOCYTE CELL-LINE, THP-1

Standard anti-leishmanial drugs were tested for their ability to inhibit the growth of intracellular amastigotes of Leishmania aethiopica, L. donovani and L. infantum in the human leukemia monocyte THP-1 cell line. Sodium stibogluconate and meglumine antimoniate were active against L. donovani with ED50 values of 8.9 mug Sb(v)/ml and 2.9 mug Sb(v)/ml, respectively. L. aethiopica was less sensitive to sodium stibogluconate with an ED50 value of 25.3 mug Sb(v)/ml while pentamidine had an ED50 value of 0.6 muM. Both L. donovani (ED50 9.3 muM), and L. aethiopica (ED50, 6.4 muM), were sensitive to aminosidine sulphate. An L. infantum isolate, clinically resistant to meglumine antimoniate treatment, had an ED50 of 22.2 mug Sb(v)/ml. The toxic level of drugs on host cells was determined by colorimetric Methyl Tetrazolium (MTT) assay prior to activity tests. The results obtained with the THP-1 in vitro drug screening model were similar to those obtained in the mouse peritoneal macrophage model.