MAP-1 AND MAP-2 BINDING-SITES AT THE C-TERMINUS OF BETA-TUBULIN - STUDIES WITH SYNTHETIC TUBULIN PEPTIDES

被引：72
|
作者：
CROSS, D
DOMINGUEZ, J
MACCIONI, RB
AVILA, J
机构：
[1] INT CTR CANC & DEV BIOL,CASILLA 70111,SANTIAGO 7,CHILE
[2] UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC,E-28049 MADRID,SPAIN
来源：
BIOCHEMISTRY | 1991年 / 30卷 / 17期
关键词：
D O I：
10.1021/bi00231a036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The interaction of microtubule-associated proteins MAP-1 and MAP-2 with different peptides containing sequences covering the C-terminal region of beta-tubulin isoforms has been analyzed. Our results indicate that MAP-1 and MAP-2 bind to a common sequence within the variable C-terminal region of the different beta-tubulin isoforms, while MAP-2 also interacts with the subdomain beta(422-434) of the constant region, in agreement with previous results (Maccioni, R. B., Rivas, C., & Vera, J. C. (1988) EMBO J. 7, 1957-1963). The productive interaction of MAP-2 with the latter domain appears to be involved in the assembly of microtubules.
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收藏
页码：4362 / 4366
页数：5
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