TRANSDUCTION OF A PLASMID CONTAINING THE BACTERIOPHAGE-D3 COS SITE IN PSEUDOMONAS-AERUGINOSA

被引:8
|
作者
SHARP, R [1 ]
GERTMAN, E [1 ]
FARINHA, MA [1 ]
KROPINSKI, AM [1 ]
机构
[1] QUEENS UNIV, DEPT MICROBIOL & IMMUNOL, KINGSTON K7L 3N6, ONTARIO, CANADA
关键词
D O I
10.1128/jb.172.6.3509-3511.1990
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Plasmids harboring the cos sequences of bacteriophage D3 can be transferred, by bacteriophage D3, into Pseudomonas aeruginosa by a mechanism which is insensitive to DNase. Transducing activity was separated from the plaque-forming particles by CsCl equilibrium gradient centrifugation. Restriction endonuclease digestion patterns suggest that the transducing particles contain plasmid concatemers.
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页码:3509 / 3511
页数:3
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