GRASS-POLLEN ALLERGENS - DETECTION ON POLLEN GRAIN SURFACE USING MEMBRANE PRINT TECHNIQUE

被引：1

作者：

ONEILL, PM ^{[1
]}

SINGH, MB ^{[1
]}

KNOX, RB ^{[1
]}

机构：

[1] UNIV MELBOURNE,SCH BOT,PARKVILLE,VIC 3052,AUSTRALIA

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | 1990年 / 91卷 / 03期

关键词：

D O I：

10.1159/000235126

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A microtechnique is described to visualize pollen allergens by making prints from the surface of mature grains on nitrocellulose membrane. The allergen Lolp I is detected by specific monoclonal antibodies or specific IgE from sera of grass-pollen-allergic patients, visualized by the immunoperoxidase method. This technique offers the potential to study the distribution of individual allergens in aerobiology samples by bright-field microscopy, and to determine the sensitivity spectra of allergic individuals to known and unknown pollen sources. © 1990 S. Karger AG, Basel.

引用

页码：266 / 269

页数：4

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