DIFFERENT MECHANISMS OF INHIBITION BY ALKYL-LYSOPHOSPHOLIPID AND PHORBOL ESTER OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR-BINDING TO HUMAN LEUKEMIC-CELL LINES

被引:0
|
作者
SHOJI, M
FUKUHARA, T
WINTON, EF
BERDEL, WE
VOGLER, WR
机构
关键词
ALKYL-LYSOPHOSPHOLIPID; PHORBOL ESTER; GM-CSF BINDING; LEUKEMIC CELLS;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We investigated the effects of rac-1-O-octadecyl-2-O-methylglycero-3-phosphocholine (ET-18-OCH3) and 12-O-tetradecanoylphorbol-13-acetate (TPA) on granulocyte-macrophage colony-stimulating factor (GM-CSF) binding to human leukemic cell lines HL60, U937, KG-1, KG-1a, and K562. HL60, U937, and KG-1 exhibited the high-affinity receptors, but KG-la and K562 revealed no demonstrable receptors. ET-inhibited 18-OCH3 inhibited GM-CSF binding to HL60, U937, and KG-1 cells with a half-maximal inhibitory concentration of 16, 10, and 78 mu M, respectively. ET-18-OCH3 at 10 mu M reduced GM-CSF binding sites on HL60, U937, and KG-1, but had little effect on the dissociation constant (Kd). ET-18-OCH3 at 10 and 30 mu M significantly (p<0.01) decreased, in a dose-depen dent manner, the total uptake, surface binding, and internalization of GM-CSF. The internalization of GM-CSF was more profoundly inhibited than its surface binding. TPA at 1 and 10 nM inhibited GM-CSF binding. Inhibition of GM-CSF binding by a combination of ET-18-OCH3 (10 mu M) and TPA (1 or 10 nM) was less than additive, and ET-18-OCH3 partially inhibited TPA-induced protein kinase C (PKC) depletion in the cytosol and translocation to the particulate fractions. It is suggested that the inhibition of GM-CSF binding by ET-18-OCH3 is due in part to disruption of the plasma membrane OCH and that the inhibition of GM-CSF binding by TPA is due to activation of PKC.
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页码:13 / 18
页数:6
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