A new subgenus of crayfish, Tubericambarus, and a new species, Cambarus (Tubericambarus) thomai, are described. The new species is most closely related to C (T.) acanthura, new combination, but differs from it in that the distomedian spine on the mesial ramus of the uropod does not overreach the rounded margin of the ramus, and the merus always has a well developed spiniform tubercle on the distroventral articular rim. The species occurs in Kentucky, Ohio, Pennsylvania, Tennessee, and West Virginia. The definition of the subgenus Lacunicambarus is amended and distributional maps of the subgenera are included.