DETERMINATION OF NICOTINE AND 2 MAJOR METABOLITES IN SERUM BY SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY PARTICLE BEAM MASS-SPECTROMETRY

被引：47
|
作者：
PACIFICI, R
PICHINI, S
ALTIERI, I
ROSA, M
BACOSI, A
CARONNA, A
ZUCCARO, P
机构：
[1] IST SUPER SANITA,VIALE REGINA ELENA 299,I-00161 ROME,ITALY
[2] OSPED SANTO SPIRITO,ROME,ITALY
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 612卷 / 02期
关键词：
D O I：
10.1016/0378-4347(93)80165-Z
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A rapid and selective assay of nicotine, cotinine and trans-3'-hydroxycotinine in human serum, based on high-performance liquid chromatography with UV detection has been developed. The compounds were subjected to solid-phase extraction, using Extrelut 1 cartridges. Recoveries were ca. 95% for nicotine, 90% for cotinine and 50-55% for trans-3'-hydroxycotinine. The limit of quantitation observed with this method was 10 ng/ml for nicotine and 5 ng/ml for each of the metabolites. The compounds were also identified using high-performance liquid chromatography with particle beam mass spectrometry, to confirm their presence in human serum.
引用
收藏
页码：209 / 213
页数：5
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