AN INTEGRAL MEMBRANE-PROTEIN FORM OF BRAIN L-GLUTAMATE DECARBOXYLASE - PURIFICATION, CHARACTERIZATION AND ITS RELATIONSHIP TO INSULIN-DEPENDENT DIABETES-MELLITUS

被引：6
|
作者：
NATHAN, B
BAO, J
HSU, CC
YAROM, M
DEUPREE, DL
LEE, YH
TANG, XW
KUO, CY
BURGHEN, GA
WU, JY
机构：
[1] UNIV KANSAS,DEPT PHYSIOL & CELL BIOL,DIV BIOL SCI,LAWRENCE,KS 66045
[2] UNIV TENNESSEE,DEPT PEDIAT,MEMPHIS,TN 38163
来源：
BRAIN RESEARCH | 1994年 / 642卷 / 1-2期
关键词：
GLUTAMIC ACID DECARBOXYLASE; INSULIN-DEPENDENT DIABETES MELLITUS; INTEGRAL MEMBRANE PROTEIN; GABA; AUTOIMMUNE DISORDER;
D O I：
10.1016/0006-8993(94)90934-2
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
A new and novel form of L-glutamate decarboxylase (GAD; EC 4.1.1.15) was purified from whole porcine brain to apparent homogeneity by a combination of column chromatographies on DE-52, ultragel AcA 34, hydroxylapatite and Sephadex G-200, and native gel electrophoresis. The purified GAD was established as an integral membrane protein based on hydrophobic interaction chromatography and membrane extraction studies. This membrane GAD (MGAD) has a native molecular weight of 120+/-5 kDa and is a homodimer of 60+/-2 kDa. Immunoprecipitation and immunoblotting tests using the sera from insulin-dependent diabetes mellitus (IDDM) patients revealed the presence of antibodies against this newly identified MGAD in IDDM. The role of MGAD in the pathogenesis of IDDM and related autoimmune disorders is also discussed.
引用
收藏
页码：297 / 302
页数：6
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