PARTIAL CHARACTERIZATION OF REACTION PRODUCTS FORMED BY DEGRADATION OF HYDALURONIC ACID WITH ASCORBIC ACID

被引：22

作者：

CLELAND, RL

STOOLMIL.AC

RODEN, L

LAURENT, TC

机构：

[1] Department of Medical Chemistry, University of Uppsala, Uppsala

[2] Department of Pediatrics and Biochemistry, the LaRabida-University of Chicago Institute and the Joseph P. Kennedy, Jr. Mental Research Center, Chicago, IL 60637, Univerity of Chicago

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 192卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0304-4165(69)90387-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hyaluronic acid was degraded with ascorbic acid at pH 3..3 for various periods of time. The extent of the degradation was measured by viscometry and by reduction of newly-generated reducing end groups with NaB3H4. Characterization of the products with respect to molecular weight distribution as well as the nature of the reducing terminal end groups showed that the degradation proceeded essentially by random cleavage of glycosidic bonds. The relative proportion of uronic acid and hexosamine end groups was determined, and it is concluded that hexosaminidic linkages were cleaved more easily than uronicidic bonds. © 1969.

引用

页码：385 / &

共 20 条

[1] THE REDUCTION OF SUGARS WITH SODIUM BOROHYDRIDE [J].