Study of Effect of Azadirachta Indica Seed Oil (Neem Seed Oil) on A Model of Pyrexia in Albino rats

To investigate the antipyretic activity of Neem Seed Oil(NSO) using Brewer's yeast induced pyrexia model. In this study the albino rats were randomly divided into groups of 6, each group consisting of 10 rats. Group I: Control (distilled water 0.5ml/rat); Group II: Standard (Paracetamol 100mg/kg intraperitoneally); Group III, IV, V, VI (0.125 ml, 0.25 ml, 0.5 ml and 1 ml/kg body weight intraperitoneally respectively). 20 % suspension of brewer's yeast was injected subcutaneously below the nape of neck to induce fever. Drugs were given after development of pyrexia and temperatures recorded. Animals showing a rise in body temperature to at least 39 degrees C were taken in the study, a total of 36 rats were taken with a minimum of 6 in each group. NSO at doses of 0.125 and 0.25 ml/kg body weight did not show any significant antipyretic effect. NSO in the dose of 0.5 ml/kg and 1ml/kg body weight showed significant (p<0.05) reduction in temperature from 2nd to 6th hour of its administration. Thus NSO exhibited significant (p<0.05) antipyretic activity at 0.5 and 1 ml/kg doses respectively.