THE 12-KDA PROTEIN OF POTATO VIRUS-M DISPLAYS PROPERTIES OF A NUCLEIC ACID-BINDING REGULATORY PROTEIN

被引：84
|
作者：
GRAMSTAT, A [1 ]
COURTPOZANIS, A [1 ]
ROHDE, W [1 ]
机构：
[1] MAX PLANCK INST ZUCHTUNGSFORSCH, W-5000 COLOGNE 30, GERMANY
来源：
FEBS LETTERS | 1990年 / 276卷 / 1-2期
关键词：
POTATO VIRUS-M; PROTEIN; 12-KDA; NUCLEIC ACID-BINDING CAPACITY; REGULATORY FACTOR;
D O I：
10.1016/0014-5793(90)80500-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The 3' terminal 1.4 kb segment of potato virus M (PVM) genomic RNA was cloned and sequenced. This part of the viral genome encodes the capsid protein CP as well as a 12 kDa protein of as yet unknown function. Both proteins were expressed in bacteria and their nucleic acid-binding properties studied. The 12 kDa protein (pr12), but not the capsid protein bound single- and double-stranded nucleic acids. This property of pr12 in conjunction with a zinc finger motif located adjacent to a basic region of the 12 kDa protein suggests that it may act as a regulatory factor during virus replication.
引用
收藏
页码：34 / 38
页数：5
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