A ONE-STEP ENZYME-IMMUNOASSAY FOR HUMAN MANGANESE SUPEROXIDE-DISMUTASE WITH MONOCLONAL-ANTIBODIES

被引：6
|
作者：
ADACHI, T
HIRANO, K
HAYASHI, K
MUTO, Y
OKUNO, F
机构：
[1] Department of Pharmaceutocs, Gifu Pharmaceutical University, Gifu, 502, 6-1
[2] First Department of Internal Medicine, Gifu University, School of Medicine, Gifu
[3] Department of Internal Medicine, Gihoku Hospital, Gifu
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 8卷 / 01期
关键词：
Clinical analysis; Enzyme immunoassay; Free radicals; Horseradish peroxidase; Manganese superoxide dismutase; Monoclonal antibody;
D O I：
10.1016/0891-5849(90)90141-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A one-step enzyme immunoassay for the determination of manganese superoxide dismutase in serum has been developed with two kinds of monoclonal antibodies. Proposed method had high sensitivity (assay range, 0.4-200 ng/ml), good recovery (recovery percentage, 102.9-106.2%) and reproducibility (intraassay, C.V. = 1.87-3.66%; interassay, C.V. = 3.03-10.4%). From these results, it is possible to apply this method to routine clinical analysis and biochemical research with various purposes. © 1990.
引用
收藏
页码：25 / 31
页数：7
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