SYNTHESIS AND FIBROBLAST GROWTH-FACTOR BINDING OF OLIGOSACCHARIDES RELATED TO HEPARIN AND HEPARAN-SULFATE

被引：20

作者：

WESTMAN, J ^{[1
]}

NILSSON, M ^{[1
]}

ORNITZ, DM ^{[1
]}

SVAHN, CM ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC BIOL & PHARMACOL,ST LOUIS,MO 63110

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 1995年 / 14卷 / 01期

关键词：

D O I：

10.1080/07328309508006439

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of six disaccharides, alpha-L-IdoA-(1-->4)-alpha-D-GlcNAc-1-->OMe alpha-L-IdoA-(1-->4)-alpha-D-GIcNSO(3)-1-->OMe, beta-D-GlcA-(1-->4)-alpha-D-GlcNAc-1-->OMe, beta-D-GlcA-(1-->4)-alpha-D-GlcNSO(3)-1-->OMe, alpha-D-GlcNAc-(1-->4)-beta-D-GlcA-1-->OMe, beta-D-GlcNAc-(1-->4)-beta-D-GlcA-1--->OMe, and two trisaccharide, beta-D-GlcA-(1-->4)-alpha-D-GlcNAc-(1-->4)-beta-D-GlcA.1-->OMe, alpha-L-IdoA-(1-->4)-alpha-D-GlcNSO(3)-(1-->4)-beta-D-GlcA-1-->Ome was prepared and screened for biological activity in vitro. The oligosaccharides were tested, togetherwith a previously synthesized trisaccharide, alpha-L-IdoA-(1-->4)-alpha-D-GlcNAc-(1-->4)-beta-D-GlcA-1-->Ome, and three tetrasaccharides, alpha-L-IdoA-(1-->4)-alpha-D-GlcNAc-(1-->4)-beta-D-GlcA-(1-->3-beta-D-Gal-1-->Ome, beta-D-GlcA-(1-->3)-beta-D-Gal-(1-->3)-beta-D-Gal-(1-->3)020PO(3)-beta-D-Xyl-1-->Ome, beta-D-GlcA-(1-->3)-beta-D-Gal-(1-->3)-beta-Gal-(1-->3)-beta-D-Xyl-1-->Ome, for competitive binding to acidic and basic fibroblast growth factor in an assay using I-125 labelled heparin. It was found that the non-sulphated trisaccharides, alpha-L-IdoA-(1-->4)-alpha-D-GlcNAc-(1-->4)-beta-D-GlcA-1-->OMe and beta-D-GlcA-(1-->4)-alpha-D-GlcNAc-(1-->4)-beta-D-GlcA-1-->OMe, and two of the disaccharides can bind to acidic as well as basic FGF.

引用

页码：95 / 113

页数：19

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