CROSS-LINKING OF SUBSTRATES OCCURS EXCLUSIVELY TO THE P66 SUBUNIT OF HETERODIMERIC HIV-1 REVERSE-TRANSCRIPTASE

被引：38

作者：

CHENG, N ^{[1
]}

PAINTER, GR ^{[1
]}

FURMAN, PA ^{[1
]}

机构：

[1] BURROUGHS WELLCOME CO,DIV ORGAN CHEM,RES TRIANGLE PK,NC 27709

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91486-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photoaffinity labeling of the hetero-and homodimeric forms of HIV-1 reverse transcriptase has been carried out using [32P]rA12-18·dT10 as a representative template-primer and [α-32P]dTTP as a representative 2′-deoxynucleoside-5′-triphosphate. UV irradiation produces stable, covalent crosslinks between each of the reactants and both the hetero-(p66/p51) and homodimeric (p66/p66,p51/p51) forms of the enzyme. In the case of the p66/p51 heterodimer, the form of the enzyme believed to be involved in viral replication, crosslinking occurs exclusively to the p66 subunit. These results suggest that the polymerase activity of the heterodimer resides on p66. © 1991.

引用

页码：785 / 789

页数：5

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