METHIONINE-REPRESSIBLE HOMOSERINE DEHYDROGENASE AND ASPARTOKINASE ACTIVITIES OF ESCHERICHIA COLI K12 - PREPARATION OF HOMOGENEOUS PROTEIN CATALYZING 2 ACTIVITEIS . MOLECULAR WEIGHT OF NATIVE ENZYME AND OF ITS SUBUNITS

被引：49
作者：
FALCOZKELLY, F
VANRAPEN.R
COHEN, GN
机构：
[1] Laboratoire d'Enzymologie du C. N. R. S. F-91, Gif-Sur-Yvette
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 8卷 / 01期
关键词：
D O I：
10.1111/j.1432-1033.1969.tb00507.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The complex protein carrying the two methionine‐repressible activities aspartokinase II and homoserine dehydrogenase II has been obtained in a homogeneous state from extracts of a genetically derepressed mutant of Escherichia coli K 12. Some general properties are described. The enzyme has a molecular weight of 169,000. In the presence of 6 M guanidine and 0.1 M mercaptoethanol, it undergoes dissociation into subunits which have a molecular weight of 43,000. The enzyme appears thus composed of four subunits of identical size. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码：146 / +
页数：1
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