3-DIMENSIONAL STRUCTURE OF HUMAN BASIC FIBROBLAST GROWTH-FACTOR, A STRUCTURAL HOMOLOG OF INTERLEUKIN-1-BETA

被引：244
作者：
ZHANG, JD
COUSENS, LS
BARR, PJ
SPRANG, SR
机构：
[1] UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST,5323 HARRY HINES BLVD,DALLAS,TX 75235
[2] CHIRON CORP,EMERYVILLE,CA 94608
[3] UNIV TEXAS,SW MED SCH,DEPT BIOCHEM,DALLAS,TX 75235
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 08期
关键词：
PROTEIN CRYSTALLOGRAPHY; HEPARIN BINDING SITE; RECEPTOR RECOGNITION;
D O I：
10.1073/pnas.88.8.3446
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The three-dimensional structure of the 146-residue form of human basic fibroblast growth factor (bFGF), expressed as a recombinant protein in yeast, has been determined by x-ray crystallography to a resolution of 1.8 angstrom. bFGF is composed entirely of beta-sheet structure, comprising a three-fold repeat of a four-stranded antiparallel beta-meander. The topology of bFGF is identical to that of interleukin 1-beta, showing that although the two proteins share only 10% sequence identity, bFGF, interleukin 1, and their homologs comprise a family of structurally related mitogenic factors. Analysis of the three-dimensional structure in light of functional studies of bFGF suggests that the receptor binding site and the positively charged heparin binding site correspond to adjacent but separate loci on the beta-barrel.
引用
收藏
页码：3446 / 3450
页数：5
相关论文
共 43 条
[1]   CHARACTERIZATION OF A CYSTEINE-FREE ANALOG OF RECOMBINANT HUMAN BASIC FIBROBLAST GROWTH-FACTOR [J].
ARAKAWA, T ;
HSU, YR ;
SCHIFFER, SG ;
TSAI, LB ;
CURLESS, C ;
FOX, GM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (01) :335-341
[2]   RECEPTOR-BINDING AND HEPARIN-BINDING DOMAINS OF BASIC FIBROBLAST GROWTH-FACTOR [J].
BAIRD, A ;
SCHUBERT, D ;
LING, N ;
GUILLEMIN, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) :2324-2328
[3]   EXPRESSION IN YEAST OF A PLASMODIUM-VIVAX ANTIGEN OF POTENTIAL USE IN A HUMAN MALARIA VACCINE [J].
BARR, PJ ;
GIBSON, HL ;
ENEA, V ;
ARNOT, DE ;
HOLLINGDALE, MR ;
NUSSENZWEIG, V .
JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (04) :1160-1171
[4]  
BARR PJ, 1988, J BIOL CHEM, V263, P16471
[5]   PROTEIN DATA BANK - COMPUTER-BASED ARCHIVAL FILE FOR MACROMOLECULAR STRUCTURES [J].
BERNSTEIN, FC ;
KOETZLE, TF ;
WILLIAMS, GJB ;
MEYER, EF ;
BRICE, MD ;
RODGERS, JR ;
KENNARD, O ;
SHIMANOUCHI, T ;
TASUMI, M .
JOURNAL OF MOLECULAR BIOLOGY, 1977, 112 (03) :535-542
[6]   CRYSTALLOGRAPHIC R-FACTOR REFINEMENT BY MOLECULAR-DYNAMICS [J].
BRUNGER, AT ;
KURIYAN, J ;
KARPLUS, M .
SCIENCE, 1987, 235 (4787) :458-460
[7]   POSSIBLE DISSOCIATION OF THE HEPARIN-BINDING AND MITOGENIC ACTIVITIES OF HEPARIN-BINDING (ACIDIC FIBROBLAST) GROWTH FACTOR-I FROM ITS RECEPTOR-BINDING ACTIVITIES BY SITE-DIRECTED MUTAGENESIS OF A SINGLE LYSINE RESIDUE [J].
BURGESS, WH ;
SHAHEEN, AM ;
RAVERA, M ;
JAYE, M ;
DONOHUE, PJ ;
WINKLES, JA .
JOURNAL OF CELL BIOLOGY, 1990, 111 (05) :2129-2138
[8]   THE HEPARIN-BINDING (FIBROBLAST) GROWTH-FACTOR FAMILY OF PROTEINS [J].
BURGESS, WH ;
MACIAG, T .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :575-606
[9]   CLONING AND EXPRESSION OF 2 DISTINCT HIGH-AFFINITY RECEPTORS CROSS-REACTING WITH ACIDIC AND BASIC FIBROBLAST GROWTH-FACTORS [J].
DIONNE, CA ;
CRUMLEY, G ;
BELLOT, F ;
KAPLOW, JM ;
SEARFOSS, G ;
RUTA, M ;
BURGESS, WH ;
JAYE, M ;
SCHLESSINGER, J .
EMBO JOURNAL, 1990, 9 (09) :2685-2692
[10]  
ECK MJ, 1989, J BIOL CHEM, V264, P17595
12345