EXTRACTION OF POLY-3-HYDROXYBUTYRATE USING CHLORINATED SOLVENTS

被引：150
|
作者：
RAMSAY, JA
BERGER, E
VOYER, R
CHAVARIE, C
RAMSAY, BA
机构：
[1] ECOLE POLYTECH,CP 6079,SUCCURSALE CENTREVILLE,MONTREAL H3C 3A7,QUEBEC,CANADA
[2] POLYFERM INC,DOLLARD ORMEAUX H9B 1P9,PQ,CANADA
来源：
BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 08期
关键词：
D O I：
10.1007/BF00152152
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Recovery of poly-3-hydroxybutyrate (PHB) in three chlorinated solvents with or without acetone pretreatment and degradation of extracted PHB (99% pure) in hot chloroform were studied. When lyophilized Alcaligenes eutrophus biomass was used, the best results were obtained with acetone pretreatment and solvent reflux for 15 min in methylene chloride or chloroform. Recovered PHB had a 95% purity and molecular weights (M(w)) of 1,050,000 and 930,000 g/mol respectively. Further heating resulted in a serious M(w) loss at reflux temperatures. Degradation of extracted PHB at 110-degrees-C in chloroform was due to random and chain-end scission, the former being predominant.
引用
收藏
页码：589 / 594
页数：6
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