NUCLEOTIDE-SEQUENCE AND GENETIC ORGANIZATION OF A 7-CENTER-DOT-3 KB REGION (MAP UNIT-47 TO 52-CENTER-DOT-5) OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS FRAGMENT ECORI-C

被引：9

作者：

KOOL, M ^{[1
]}

BROER, R ^{[1
]}

ZUIDEMA, D ^{[1
]}

GOLDBACH, RW ^{[1
]}

VLAK, JM ^{[1
]}

机构：

[1] WAGENINGEN UNIV AGR,DEPT VIROL,6709 PD WAGENINGEN,NETHERLANDS

来源：

JOURNAL OF GENERAL VIROLOGY | 1994年 / 75卷

关键词：

D O I：

10.1099/0022-1317-75-3-487

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nucleotide sequence and genetic organization of a 7297 bp region within the EcoRI-C fragment of Autographia-californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) are presented. Eight putative open reading frames were found and their respective amino acid sequences compared with a number of data libraries. ORF 1227 corresponded with gp41 and its predicted protein sequence was found to be 55 amino acids longer at its C terminus than reported previously. Moreover the main part of the ORF 1227 product, including the additional 55 amino acids, showed a high degree of homology with protein p40 of Helicoverpa-zea single nucleocapsid nuclear polyhedrosis virus (HzSNPV). Three other ORFs in the analysed AcMNPV region showed homology with ORFs in the HzSNPV sequence, indicating that the general organization of this region is similar in both viruses. However one ORF found in the AcMNPV sequence was absent from the corresponding HzSNPV sequence.

引用

页码：487 / 494

页数：8