EXPRESSION OF THE BETA-GLUCURONIDASE GENE IN POLLEN OF LILY (LILIUM-LONGIFLORUM), TOBACCO (NICOTIANA-TABACUM), NICOTIANA-RUSTICA, AND PEONY (PEONIA-LACTIFLORA) BY PARTICLE BOMBARDMENT

被引：55

作者：

NISHIHARA, M

ITO, M

TANAKA, I

KYO, M

ONO, K

IRIFUNE, K

MORIKAWA, H

机构：

[1] HIROSHIMA UNIV,FAC SCI,DEPT GENE SCI,HIROSHIMA 724,JAPAN

[2] YOKOHAMA CITY UNIV,DEPT BIOL,YOKOHAMA 236,JAPAN

[3] KUMAMOTO UNIV,FAC SCI,DEPT BIOL,KUMAMOTO 860,JAPAN

[4] KAGAWA UNIV,FAC AGR,DEPT BIORESOURCE SCI,MIKI,KAGAWA 76107,JAPAN

[5] SHIZUOKA UNIV,FAC SCI,DEPT BIOL,OYA,SHIZUOKA 422,JAPAN

来源：

PLANT PHYSIOLOGY | 1993年 / 102卷 / 02期

关键词：

D O I：

10.1104/pp.102.2.357

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A beta-glucuronidase (GUS) gene that is under the control of the anther-specific LAT52 promoter of tomato (Lycopersicon esculentum) and the nopaline synthetase polyadenylation terminator was successfully expressed in pollen of Lilium longiflorum, Nicotiana tabacum, Nicotiana rustica, and Paeonia lactiflora using a pneumatic particle gun. The GUS gene in plasmid pBI221 was also expressed, to a lesser extent, in pollen of all of these species. The presence of methanol in the substrate solution for histochemical GUS assay and the incubation time in this solution influenced successful detection of GUS expression in bombarded pollen. Cytological analysis of GUS-expressing pollen of lily showed that introduced gold particles were seen in intracellular compartments of pollen, including the vegetative cytoplasm, vegetative nucleus, and generative cytoplasm.

引用

页码：357 / 361

页数：5