SOLUBILIZATION OF RAT-KIDNEY LYSOSOMES IN REVERSED MICELLES OF AEROSOL OT IN OCTANE

被引：1

作者：

PSHEZHETSKY, AV ^{[1
]}

BUNEEVA, OA ^{[1
]}

WIEDERSCHAIN, GY ^{[1
]}

机构：

[1] ACAD MED SCI USSR, INST BIOL & MED CHEM, MOSCOW 109801, USSR

来源：

FEBS LETTERS | 1991年 / 287卷 / 1-2期

关键词：

LYSOSOMAL GLYCOSIDASE; N-ACETYL-BETA-D-HEXOSAMINIDASE; MICELLAR ENZYMOLOGY; REVERSED MICELLE;

D O I：

10.1016/0014-5793(91)80055-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intact lysosomes from rat kidneys were solubilized in the ternary system: surfactant (Aerosol OT)-buffer-organic solvent. According to data of laser light-scattering analysis and kinetic experiments with the lysosomal marker enzyme, N-acetyl-beta-D-hexosaminidase (EC 3.2.1.30), the solubilization of lysosomes in this system resulted in the destruction of the lysosomes and the entrapping of their components in reversed micelles.

引用

页码：219 / 222

页数：4

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