TETHERED BANDSHIFT ASSAY AND AFFINITY PURIFICATION OF A NEW DNA-BINDING PROTEIN

被引：0

作者：

REN, LF ^{[1
]}

CHEN, H ^{[1
]}

STERNBERG, EA ^{[1
]}

机构：

[1] MED COLL WISCONSIN,DEPT PHYSIOL,MILWAUKEE,WI 53226

来源：

BIOTECHNIQUES | 1994年 / 16卷 / 05期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA-binding proteins can be purified by their affinity for probes with specific sequences that are tethered to magnetic beads. A restriction site at the end of the tether allows release of probe with factor still on it. This probe with bound factor can be bandshifted directly to compare to bandshifts in whole extracts. This method correlates purified factor seen indirectly by bandshift with factor that is purified and released because they are the same.

引用

页码：852 / 855

页数：4

共 50 条

[1] Affinity purification of DNA-binding proteins
Gadgil, H
Oak, SA
Jarrett, HW
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 49 (1-3): : 607 - 624
[2] A NEW FAST AND EASY METHOD FOR THE OLIGONUCLEOTIDE AFFINITY PURIFICATION OF DNA-BINDING PROTEINS
KIRCH, HC
KRUGER, H
HOLTHAUSEN, HS
NUCLEIC ACIDS RESEARCH, 1991, 19 (11) : 3156 - 3156
[3] A NEW AFFINITY PURIFICATION PROCEDURE FOR DNA-BINDING PROTEINS USING BROMOACETYL AGAROSE
KANG, SH
XU, X
HEIDENREICH, O
GRYAZNOV, S
NERENBERG, M
NUCLEIC ACIDS RESEARCH, 1995, 23 (12) : 2344 - 2345
[4] Purification and characterization of LasR as a DNA-binding protein
You, ZY
Fukushima, J
Ishiwata, T
Chang, BT
Kurata, M
Kawamoto, S
Williams, P
Okuda, K
FEMS MICROBIOLOGY LETTERS, 1996, 142 (2-3) : 301 - 307
[5] THE PURIFICATION AND DNA-BINDING ACTIVITY OF AN ULTRABITHORAX PROTEIN
GAVIS, E
KRASNOW, M
BEACHY, P
HOGNESS, D
JOURNAL OF CELLULAR BIOCHEMISTRY, 1987, : 105 - 105
[6] AFFINITY PURIFICATION OF SEQUENCE-SPECIFIC DNA-BINDING PROTEINS
KADONAGA, JT
TJIAN, R
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (16) : 5889 - 5893
[7] Immobilized DNA-binding assay, an approach for in vitro DNA-binding assay
Kim, JH
ANALYTICAL BIOCHEMISTRY, 2004, 334 (02) : 401 - 402
[8] PURIFICATION OF DNA-BINDING PROTEINS USING TANDEM DNA-AFFINITY COLUMN
OSTROWSKI, J
BOMSZTYK, K
NUCLEIC ACIDS RESEARCH, 1993, 21 (04) : 1045 - 1046
[9] AFFINITY LATEX FOR THE PURIFICATION OF SEQUENCE-SPECIFIC DNA-BINDING PROTEINS
KAWAGUCHI, H
INOMATA, Y
HANDA, H
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1991, 201 : 334 - COLL
[10] A NONRADIOACTIVE ASSAY FOR THE DETECTION AND QUANTITATION OF A DNA-BINDING PROTEIN
HIBMA, MH
ELY, SJ
CRAWFORD, L
NUCLEIC ACIDS RESEARCH, 1994, 22 (18) : 3806 - 3807

← 1 2 3 4 5 →