URINARY-EXCRETION OF DEUTERIUM-LABELED FOLATE AND THE METABOLITE P-AMINOBENZOYLGLUTAMATE IN HUMANS

被引:0
|
作者
KOWNACKIBROWN, PA [1 ]
WANG, CZ [1 ]
BAILEY, LB [1 ]
TOTH, JP [1 ]
GREGORY, JF [1 ]
机构
[1] UNIV FLORIDA, DEPT FOOD SCI & HUMAN NUTR, GAINESVILLE, FL 32611 USA
来源
JOURNAL OF NUTRITION | 1993年 / 123卷 / 06期
关键词
FOLATE; STABLE ISOTOPE; HUMANS; P-AMINOBENZOYLGLUTAMATE;
D O I
暂无
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Stable isotopic methods were employed to determine the proportion of ingested folate excreted as the metabolite p-aminobenzoylglutamate (pABG, free and N-acetyl) in urine. Adult male subjects (n = 7) were maintained on a 4.54 mumol/d (2 mg/d) folate saturation regimen. After 7 d, subjects were given a mixed oral dose containing 0.67 mumol (300 mug) each of bideuterofolic acid (d2) and tetradeutero-folic acid (d4). Urine was collected for the following 48 h and analyzed for folate and pABG. The extent of deuterium labeling of urinary folate and pABG was determined by gas chromatography-mass spectrometry. Urinary total pABG excretion increased less than twofold as a result of the folate saturation, whereas urinary folate increased over 10-fold. Urinary d2 and d4 folates each contained 14-15% of the respective oral doses of labeled compounds, whereas urinary d2 and d4 pABG comprised only 0.98-1.15% of the labeled doses. Molar ratios (d2/d4) of excreted folate and pABG indicated that there was no in vivo isotopic discrimination between the labeled folates. Urinary pABG accounted for 5.1 +/- 0.6% of total ingested folate, whereas labeled pABG was about 6.7-7.6% of the excretion of labeled compounds (i.e., labeled folate + pABG). This study indicated that pABG is not a major excretory product during folate supplementation, but its relative importance may increase in conditions of reduced folate nutriture.
引用
收藏
页码:1101 / 1108
页数:8
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