COLLECTION OF EGGS AND HATCHING AND CULTURING 2ND-STAGE LARVAE OF TOXOCARA-VITULORUM INVITRO

被引：14
|
作者：
RAJAPAKSE, RPVJ
VASANTHATHILAKE, VWSM
LLOYD, S
FERNANDO, ST
机构：
[1] UNIV CAMBRIDGE,DEPT CLIN VET MED,MADINGLEY RD,CAMBRIDGE CB3 0ES,ENGLAND
[2] UNIV PERADENIYA,FAC VET MED & ANIM SCI,PERADENIYA,SRI LANKA
来源：
JOURNAL OF PARASITOLOGY | 1992年 / 78卷 / 06期
关键词：
D O I：
10.2307/3283237
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Eggs of Toxocara vitulorum were harvested from the feces of infected buffalo calves and embryonated in vitro. Optimum conditions for hatch and culture of the second-stage larvae were determined. Maximum hatch of larvae occurred from eggs that were decoated by treatment with saturated Ca(OCl)2 for 1624 min followed by treatment with CO2 and incubation at 37 C. Larvae could be cultured in RPMI-1640 medium for up to 3 mo but survived for only 3 wk in Eagle's minimum essential medium.
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页码：1090 / 1092
页数：3
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