SCANNING SLIT APERTURE CONFOCAL MICROSCOPY FOR 3-DIMENSIONAL IMAGING

被引：6
|
作者：
BURNS, DH
HATANGADI, RB
SPELMAN, FA
机构：
[1] Center for Bioengineering, University of Washington, Seattle, Washington
来源：
SCANNING | 1990年 / 12卷 / 03期
关键词：
D O I：
10.1002/sca.4950120307
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
A scanning confocal microscope using stationary slit apertures of variable width is described. Scanning is achieved by employing an oscillating mirror galvanometer capable of scanning images at 160 frames/s. The microscope depth‐of‐focus is characterized for several slit widths and shown to approach diffraction‐limited performance as width decreases. Reduction of flare in images taken with narrow slit widths is demonstrated. In addition, the optical sectioning capability of the system is demonstrated using polymer casts of the vasculature of rat kidney. Copyright © 1990 Foundation for Advances in Medicine and Science, Inc.
引用
收藏
页码：156 / 160
页数：5
相关论文
共 50 条
  • [21] Scanning slit confocal microscopy of crystalline keratitis
    Auran, JD
    Shayegani, A
    Donnenfeld, ED
    Perry, HD
    Grossman, G
    Zaidman, GW
    Konariek, JP
    Koester, CJ
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1996, 37 (03) : 4709 - 4709
  • [22] Scanning slit confocal microscopy of fungal keratitis
    Florakis, GJ
    Moazami, G
    Schubert, H
    Koester, CJ
    Auran, JD
    ARCHIVES OF OPHTHALMOLOGY, 1997, 115 (11) : 1461 - 1463
  • [23] 3-DIMENSIONAL VISUALIZATION TECHNIQUES FOR CONFOCAL MICROSCOPY
    VANDERVOORT, HTM
    BRAKENHOFF, GJ
    NANNINGA, N
    ULTRAMICROSCOPY, 1988, 24 (04) : 451 - 451
  • [24] 3-DIMENSIONAL DIGITAL CONFOCAL RAMAN MICROSCOPY
    GOVIL, A
    PALLISTER, DM
    MORRIS, MD
    APPLIED SPECTROSCOPY, 1993, 47 (01) : 75 - 79
  • [25] INTRODUCTION TO CONFOCAL MICROSCOPY AND 3-DIMENSIONAL RECONSTRUCTION
    WRIGHT, SJ
    CENTONZE, VE
    STRICKER, SA
    DEVRIES, PJ
    PADDOCK, SW
    SCHATTEN, G
    METHODS IN CELL BIOLOGY, VOL 38: CELL BIOLOGICAL APPLICATIONS OF CONFOCAL MICROSCOPY, 1993, 38 : 1 - 45
  • [26] CONFOCAL FLUORESCENCE MICROSCOPY AND 3-DIMENSIONAL RECONSTRUCTION
    WRIGHT, SJ
    SCHATTEN, G
    JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1991, 18 (01): : 2 - 10
  • [27] 3-DIMENSIONAL VISUALIZATION METHODS FOR CONFOCAL MICROSCOPY
    VANDERVOORT, HTM
    BRAKENHOFF, GJ
    BAARSLAG, MW
    JOURNAL OF MICROSCOPY-OXFORD, 1989, 153 : 123 - 132
  • [28] 3-DIMENSIONAL PROBES IN CONFOCAL SCANNING LIGHT-MICROSCOPY USING OPTICAL SECTIONS
    HOWARD, V
    JOURNAL OF ANATOMY, 1986, 149 : 212 - 212
  • [29] 3-DIMENSIONAL LASER-SCANNING CONFOCAL MICROSCOPY OF IN-SITU HYBRIDIZATION IN THE SKIN
    MAHONEY, SE
    PADDOCK, SW
    SMITH, LC
    LEWIS, DE
    DUVIC, M
    AMERICAN JOURNAL OF DERMATOPATHOLOGY, 1994, 16 (01) : 44 - 51
  • [30] 3-DIMENSIONAL RECONSTRUCTION OF THE RABBIT CORNEA BY CONFOCAL SCANNING OPTICAL MICROSCOPY AND VOLUME RENDERING
    MASTERS, BR
    PADDOCK, SW
    APPLIED OPTICS, 1990, 29 (26): : 3816 - 3822
12345