URINE MUTAGENICITY AND BIOCHEMICAL PARAMETERS AS MARKERS OF EXPOSURE TO PETROLEUM PITCH USING A RAT MODEL

A petroleum pitch sample collected in a carbon electrode factory was studied using a series of in vivo assays for genotoxicity and enzymatic induction capability. Rats were treated with the petroleum derivative in three doses: 100, 50, and 10 mg/kg body weight. The treatment produced a rapid excretion of mutagenic substances in the urines of the first 24 hr only in rats treated with high doses (100 and 50 mg/kg). No faecal mutagenic activity was observed. Analyses of urinary thioethers showed that urinary metabolites derived from the compounds present in the pitchsample at the lowest doseadministered (10 mg/kg) were eliminated primarily as cysteine conjugates. The pitch sample was found to be a good inducer of pulmonary and hepatic aryl hydrocarbon hydroxylase, especially after a 50 mg/kg dose. Urinary D‐glucaric acid content was always statistically increased in treated animals compared with controls, confirming the enzymatic induction activity. Hepatic glutathione‐S‐transferase activity increased following treatment with 50 and 10 mg/kg doses. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company