Vaccine Candidate Against COVID-19 Based on Structurally Modified Plant Virus as an Adjuvant

被引：10
|
作者：
Kovalenko, Angelina O. [1 ]
Ryabchevskaya, Ekaterina M. [1 ]
Evtushenko, Ekaterina A. [1 ]
Manukhova, Tatiana I. [1 ]
Kondakova, Olga A. [1 ]
Ivanov, Peter A. [1 ]
Arkhipenko, Marina V. [1 ]
Gushchin, Vladimir A. [1 ,2 ]
Nikitin, Nikolai A. [1 ]
Karpova, Olga V. [1 ]
机构：
[1] Lomonosov Moscow State Univ, Fac Biol, Dept Virol, Moscow, Russia
[2] Minist Hlth Russian Federat, NF Gamaleya Natl Res Ctr Epidemiol & Microbiol, Moscow, Russia
来源：
FRONTIERS IN MICROBIOLOGY | 2022年 / 13卷
基金：
俄罗斯基础研究基金会;
关键词：
coronaviruses; vaccine candidate; plant virus; adjuvant; tobacco mosaic virus; structurally modified plant virus; RECEPTOR-BINDING DOMAIN; TOBACCO-MOSAIC-VIRUS; SARS-COV-2; SPIKE; SPHERICAL-PARTICLES; SARS-COV; PROTEIN; MUTATIONS; INFECTION; EPITOPES; VIRIONS;
D O I：
10.3389/fmicb.2022.845316
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A recombinant vaccine candidate has been developed based on the major coronaviruses' antigen (S protein) fragments and a novel adjuvant-spherical particles (SPs) formed during tobacco mosaic virus thermal remodeling. The receptor-binding domain and the highly conserved antigenic fragments of the S2 protein subunit were chosen for the design of recombinant coronavirus antigens. The set of three antigens (Co1, CoF, and PE) was developed and used to create a vaccine candidate composed of antigens and SPs (SPs + 3AG). Recognition of SPs + 3AG compositions by commercially available antibodies against spike proteins of SARS-CoV and SARS-CoV-2 was confirmed. The immunogenicity testing of these compositions in a mouse model showed that SPs improved immune response to the CoF and PE antigens. Total IgG titers against both proteins were 9-16 times higher than those to SPs. Neutralizing activity against SARS-CoV-2 in serum samples collected from hamsters immunized with the SPs + 3AG was demonstrated.
引用
收藏
页数：15
相关论文
共 50 条
  • [41] Oxytocin as a Potential Adjuvant Against COVID-19 Infection
    Thakur, Pratibha
    Shrivastava, Renu
    Shrivastava, Vinoy K.
    ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS, 2021, 21 (07) : 1155 - 1162
  • [42] Antithrombotic drugs with adjuvant action against COVID-19
    Di Micco, Pierpaolo
    Di Micco, Gianluca
    Russo, Vincenzo
    Fontanella, Andrea
    ITALIAN JOURNAL OF MEDICINE, 2020, 14 (04) : 241 - 244
  • [43] QadirVax-19: A multi epitope-based vaccine against COVID-19
    Mukhtar, Misha
    Qadir, Muhammad I.
    KUWAIT JOURNAL OF SCIENCE, 2023, 50 (01)
  • [44] The granting of emergency use designation to COVID-19 candidate vaccines: implications for COVID-19 vaccine trials
    Singh, Jerome Amir
    Upshur, Ross E. G.
    LANCET INFECTIOUS DISEASES, 2021, 21 (04): : E103 - E109
  • [45] Covid-19: WHO warns against "vaccine nationalism" or face further virus mutations
    Eaton, Lynn
    BMJ-BRITISH MEDICAL JOURNAL, 2021, 372 : n292
  • [46] Low toxicity and high immunogenicity of an inactivated vaccine candidate against COVID-19 in different animal models
    Wang, Ze-Jun
    Zhang, Hua-Jun
    Lu, Jia
    Xu, Kang-Wei
    Peng, Cheng
    Guo, Jing
    Gao, Xiao-Xiao
    Wan, Xin
    Wang, Wen-Hui
    Shan, Chao
    Zhang, Su-Cai
    Wu, Jie
    Yang, An-Na
    Zhu, Yan
    Xiao, Ao
    Zhang, Lei
    Fu, Lie
    Si, Hao-Rui
    Cai, Qian
    Yang, Xing-Lou
    You, Lei
    Zhou, Yan-Ping
    Liu, Jing
    Pang, De-Qing
    Jin, Wei-Ping
    Zhang, Xiao-Yu
    Meng, Sheng-Li
    Sun, Yun-Xia
    Desselberger, Ulrich
    Wang, Jun-Zhi
    Li, Xin-Guo
    Duan, Kai
    Li, Chang-Gui
    Xu, Miao
    Shi, Zheng-Li
    Yuan, Zhi-Ming
    Yang, Xiao-Ming
    Shen, Shuo
    EMERGING MICROBES & INFECTIONS, 2020, 9 (01) : 2606 - 2618
  • [47] Bioinformatics Analysis of SARS-CoV-2 to Approach an Effective Vaccine Candidate Against COVID-19
    Seyed Mehdi Sadat
    Mohammad Reza Aghadadeghi
    Masoume Yousefi
    Arezoo Khodaei
    Mona Sadat Larijani
    Golnaz Bahramali
    Molecular Biotechnology, 2021, 63 : 389 - 409
  • [48] Bioinformatics Analysis of SARS-CoV-2 to Approach an Effective Vaccine Candidate Against COVID-19
    Sadat, Seyed Mehdi
    Aghadadeghi, Mohammad Reza
    Yousefi, Masoume
    Khodaei, Arezoo
    Sadat Larijani, Mona
    Bahramali, Golnaz
    MOLECULAR BIOTECHNOLOGY, 2021, 63 (05) : 389 - 409
  • [49] Preclinical immunogenicity and efficacy of a candidate COVID-19 vaccine based on a vesicular stomatitis virus-SARS-CoV-2 chimera
    Espeseth, Amy S.
    Yuan, Maoli
    Citron, Michael
    Reiserova, Lucia
    Morrow, Gavin
    Wilson, Aaron
    Horton, Melanie
    Rukhman, Mark
    Kinek, Keith
    Hou, Fuxiang
    Li, Shui L.
    Li, Fengsheng
    Choi, Yesle
    Heidecker, Gwen
    Luo, Bin
    Wu, Guoxin
    Zhang, Lan
    Strable, Erica
    DeStefano, Joanne
    Secore, Susan
    Mukhopadhyay, Tarit K.
    Richardson, Douglas D.
    Sayeed, Eddy
    Welch, Lisa S.
    Bett, Andrew J.
    Feinberg, Mark B.
    Gupta, Swati B.
    Cooper, Christopher L.
    Parks, Christopher L.
    EBIOMEDICINE, 2022, 82
  • [50] Efficacy and Safety of a Recombinant Plant-Based Adjuvanted Covid-19 Vaccine
    Hager, Karen J.
    Perez Marc, Gonzalo
    Gobeil, Philipe
    Diaz, Ricardo S.
    Heizer, Gretchen
    Llapur, Conrado
    Makarkov, Alexander I.
    Vasconcellos, Eduardo
    Pillet, Stephane
    Riera, Fernando
    Saxena, Pooja
    Geller Wolff, Priscila
    Bhutada, Kapil
    Wallace, Garry
    Aazami, Hessam
    Jones, Christine E.
    Polack, Fernando P.
    Ferrara, Luciana
    Atkins, Judith
    Boulay, Iohann
    Dhaliwall, Jiwanjeet
    Charland, Nathalie
    Couture, Manon M. J.
    Jiang-Wright, Julia
    Landry, Nathalie
    Lapointe, Sophie
    Lorin, Aurelien
    Mahmood, Asif
    Moulton, Lawrence H.
    Pahmer, Emily
    Parent, Julie
    Seguin, Annie
    Tran, Luan
    Breuer, Thomas
    Ceregido, Maria-Angeles
    Koutsoukos, Marguerite
    Roman, Francois
    Namba, Junya
    D'Aoust, Marc-Andre
    Trepanier, Sonia
    Kimura, Yosuke
    Ward, Brian J.
    NEW ENGLAND JOURNAL OF MEDICINE, 2022, 386 (22): : 2084 - 2096
12345