A Rapid, Label-free and Impedimetric DNA Sensor Based on PNA-modified Nanoporous Gold Electrode

被引：5
|
作者：
Tao, Wenyan [1 ,2 ,3 ,4 ]
Lin, Peng [1 ,2 ]
Liu, Hong [1 ,2 ]
Ke, Shanming [1 ,2 ]
Zeng, Xierong [1 ,2 ]
机构：
[1] Shenzhen Univ, Shenzhen Key Lab Special Funct Mat, Coll Mat Sci & Engn, Shenzhen, Peoples R China
[2] Shenzhen Univ, Shenzhen Engn Lab Adv Technol Ceram, Coll Mat Sci & Engn, Shenzhen, Peoples R China
[3] Shenzhen Univ, Key Lab Optoelect Devices & Syst, Minist Educ, Shenzhen, Guangdong, Peoples R China
[4] Shenzhen Univ, Coll Optoelect Engn, Shenzhen, Guangdong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2017年 / 12卷 / 11期
关键词：
anodic aluminum oxide; nanoporous gold electrode; peptide nucleic acid; DNA sensing; ANODIC ALUMINUM-OXIDE; SINGLE-NUCLEOTIDE MISMATCHES; POROUS ALUMINA; ELECTROCHEMICAL DETECTION; 2-DIMENSIONAL NETWORKS; HYBRIDIZATION; ACID; NANOPARTICLES; SPECTROSCOPY; NANORODS;
D O I：
10.20964/2017.11.38
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A gold nanoparticle film was successfully prepared on highly ordered and porous anodic aluminum oxide substrate through thermal evaporation deposition technique. As-fabricated nanoporous gold electrode modified by thiol-derivative peptide nucleic acid (PNA) was applied for impedimetric DNA sensing. In-situ kinetic analysis of PNA/DNA hybridization process was realized by electrochemical impedance spectroscopy (EIS) method. Considering the special structure of nanoporous gold electrode, a new equivalent circuit model was proposed for EIS data fitting. The sensitivity of PNA-modified nanoporous gold electrode is 0.4 Omega/cm(2).nM. Meanwhile, complementary DNA target could be detected at concentration as low as 10 nM, which indicating that a methodology for rapid, sensitive and label-free detection of DNA has been set-up based on nanoporous gold electrode.
引用
收藏
页码：10511 / 10523
页数：13
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