Membrane Protein Structure Determination using Paramagnetic Tags

被引：8

作者：

Ganguly, Soumya ^{[1
,2
,3
]}

Weiner, Brian E. ^{[1
,2
,3
]}

Meiler, Jens ^{[1
,2
,3
]}

机构：

[1] Vanderbilt Univ, Inst Biol Chem, Struct Biol Ctr, Dept Chem, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Inst Biol Chem, Struct Biol Ctr, Dept Pharmacol, Nashville, TN 37235 USA

[3] Vanderbilt Univ, Inst Biol Chem, Struct Biol Ctr, Dept Biomed Informat, Nashville, TN 37235 USA

来源：

STRUCTURE | 2011年 / 19卷 / 04期

基金：

美国国家科学基金会;

关键词：

NUCLEAR-MAGNETIC-RESONANCE; CONSTRAINTS; PREDICTION; NMR;

D O I：

10.1016/j.str.2011.03.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The combination of paramagnetic tagging strategies with NMR or EPR spectroscopic techniques can revolutionize de novo structure determination of helical membrane proteins. Leveraging the full potential of this approach requires optimal labeling strategies and prediction of membrane protein topology from sparse and low-resolution distance restraints, as addressed by Chen et al. (2011).

引用

页码：441 / 443

页数：3

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