Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans

A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01(T) was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening process from food-related sources. IRB4-01(T) grew at 10-50 degrees C (optimum, 35-37 degrees C), pH 6-10 (optimum, pH 7) and in 0-6% NaCl (optimum, 1-3 %). Phylogenetic analyses based on 16S rRNA and combined short- and long-read genome sequencing revealed that IRB4-01(T) is closely related to Lysinibacillus cavernae SYSU K30005(T) and Lysinibacillus boronitolerans 10a(T). The cell-wall peptidoglycan type was A4 alpha (Lys-Asp), containing the diagnostic diamino acid lysine. The major polar lipids in strain IRB4-01(T) were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid, while the predominant menaquinone was MK-7. The major cellular fatty acids were iso-C-15:0, anteiso-C-15:0 and iso-C-16:0. Genomic DNA G+C content was 37.4 mol%, while the digital DNA-DNA hybridization revealed 33.6 and 32.3% relatedness to L. cavernae SYSU K30005(T) and L. boronitolerans 10a(T), respectively. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome comparisons, strain IRB4-01(T) represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillus irui sp. nov. is proposed. The type strain is IRB4-01(T) (NCIMB 15452(T)=LMG 32887(T)). Hybrid genome data are provided on the NCBI database using the Bioproject number PRJNA906010 and accession numbers CP113527 and CP113528. Additionally, a representative 16S rRNA sequence is available with the GenBank accession number OQ566940.