RNase H-dependent DNA thresholder modulated by cancer marker concentration

被引：0
|
作者：
Nour, Moustapha A. Y. [1 ]
Drozd, Valeriia S. [1 ]
Lemeshko, Evgenii A. [1 ]
Tafran, Lilia [1 ]
Salimova, Adeliia A. [1 ]
Kulikova, Alexandra V. [1 ]
Eldeeb, Ahmed A. [1 ]
机构：
[1] SCAMT Inst ITMO Univ, Lab Nucl Acid Nanotechnol, 9 Lomonosova Str, St Petersburg 191002, Russia
来源：
CHEMICAL COMMUNICATIONS | 2024年 / 60卷 / 33期
关键词：
CLUSTER;
D O I：
10.1039/d4cc00495g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations. The mRNA cleavage is activated by 2.6, 7.5 or 39.5 nM of biomarker depending on the construct design. The constructs can be used to differentiate cancer from normal cells by the level of oncogene expression followed by silencing of a targeted gene. Threshold antisense oligonucleotide constructs were designed to cleave mRNA within different biomarker concentrations.
引用
收藏
页码：4427 / 4430
页数：4
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